B.Pharma Important Question
BP-605T,
Pharmaceutical Biotechnology,
VI Sem, RGPV, B.Pharmacy
UNIT I
Q.1 Discuss the various techniques for enzyme immobilization. (RGPV May 2024)
Q.2 Explain in detail the types and working principle of biosensor with examples. (RGPV May 2024)
Q.3 Discuss the production and industrial application of Protease. (RGPV May 2024)
Q.4 Discuss the basic concept and brief introduction of biotechnology in pharmaceutical sciences. (RGPV Nov 2023)
Q.5 Describe techniques used in protein engineering. (RGPV Nov 2023)
Q.6 Explain the production and industrial application of amylase. (RGPV Nov 2023)
Q.7 What is enzyme immobilization? Write its methods and applications. (RGPV May 2022)
Q.8 Define protein engineering. Write principle and applications of genetics engineering. (RGPV May 2022)
UNIT II
Q.1 Explain in detail Restriction endonuclease and DNA ligase. (RGPV May 2024)
Q.2 Describe the production of Hepatitis-B vaccine by rDNA technology. (RGPV May 2024)
Q.3 Discuss steps involved in Polymerase Chain Reaction (PCR). (RGPV May 2024)
Q.4 Discuss plasmids as cloning vector. (RGPV Nov 2023)
Q.5 Discuss the production of Insulin by rDNA technology. (RGPV Nov 2023)
Q.6 Define rDNA technology. Write application of genetic engineering. (RGPV Nov 2023)
Q.7 Write an exhaustive note on Recombinant DNA technology and its application. (RGPV May 2022)
Q.8 Write a brief introduction to PCR. (RGPV May 2022)
Q.9 Write short notes on any two. (RGPV May 2022)
a) Storage condition and stability of official vaccines
b) Production of Penicillin
c) Structure of Immunoglobulin
UNIT III
Q.1 Describe in detail step-wise production process of Bacterial Vaccine. (RGPV May 2024)
Q.2 Explain the production of Monoclonal antibodies with their applications. (RGPV May 2024)
Q.3 Write structure and functions of Major Histo compatibility Complex. (RGPV May 2024)
Q.4 Discuss general method of the preparation of toxoids. (RGPV Nov 2023)
Q.5 Explain different types of hypersensitivity reaction with examples. (RGPV Nov 2023)
Q.6 Discuss storage conditions and stability of official vaccines. (RGPV Nov 2023)
Q.7 Explain steps involved in western blotting technique. (RGPV Nov 2023)
Q.8 Structure and functions of MHC. (RGPV May 2022)
Q.9 Discuss in detail about blood product and plasma substitutes with special reference to stability and storage condition. (RGPV May 2022)
UNIT IV
Q.1 Explain steps involved in southern blotting technique. (RGPV May 2024)
Q.2 Explain the types and different methods of isolation of mutants. (RGPV May 2024)
Q.3 Describe Bacterial Conjugation process. (RGPV May 2024)
Q.4 Discuss genetic organization of eukaryotes and prokaryotes. (RGPV Nov 2023)
Q.5 Describe bacterial transformation process with diagram. (RGPV Nov 2023)
UNIT V
Q.1 Explain production of penicillin by fermentation. (RGPV May 2024)
Q.2 Write in brief collection, processing and storage of whole human blood. (RGPV May 2024)
Q.3 Explain production of vitamin B₁₂ by fermentation. (RGPV Nov 2023)
Q.4 Write in brief collection, processing and storage of dried human plasma. (RGPV Nov 2023)
Q.5 What is fermentation? Explain in brief large scale production fermenter design and its control. (RGPV May 2022)
Q.6 Briefly explain collection, processing and storage of dried human plasma and plasma substitutes. (RGPV May 2022)
Extra Questions
Q.1 Write short notes on any two: (RGPV May 2024)
a) Fermentation methods and general requirements
b) Principles of genetic engineering
c) Blood products and Plasma Substitutes
d) ELISA
Q.2 Write short notes on any two: (RGPV Nov 2023)
a) Physical entrapment and covalent attachment
b) Interferon
c) Immunoglobulins
d) Mutation
— Best of Luck for Exam —
