B.Pharma Important Question
BP-303T,
Pharmaceutical Microbiology,
III Sem, RGPV, B.Pharmacy
UNIT 1 – History of Microbiology
Q.1 Explain the raw materials used for bacteria culture media. (RGPV Dec 2023)
Q.2 Discuss various types of electron microscopy. (RGPV Dec 2023)
Q.3 Classify Bacteria. Explain their cultivation and reproduction along with their life cycle in human beings. Discuss the various disease caused by Bacteria. (RGPV June 2020)
Q.4 Give an account of the scope of different branches of microbiology indicating modern development in each branch. Give an account of electron microscope and its working. (RGPV June 2020)
Q.5 Explain Growth Curve. Discuss various pure culture techniques. (RGPV Nov 2019)
UNIT 2 – Identification of Bacteria
Q.1 What are various pure culture techniques in bacteria? (RGPV Dec 2023)
Q.2 Explain the principle and procedure of acid fast staining. (RGPV Dec 2023)
Q.3 Describe the principle and procedure of Moist heat sterilization. (RGPV Dec 2023)
Q.4 Explain Biochemical tests used for the identification of bacteria. (RGPV Dec 2023)
Q.5 Enumerate various methods of Sterilization. Describe sterilization by radiation in detail giving suitable examples. (RGPV June 2020)
Q.6 Define Sterilization. Explain physical method of sterilization and its pharmaceutical applications. (RGPV Nov 2019)
Q.7 Give morphological classification of bacteria. Explain Grams staining techniques. (RGPV Nov 2019)
UNIT 3 – Study of Morphology
Q.1 Discuss reproduction in Viruses with a suitable diagram. (RGPV Dec 2023)
Q.2 Discuss the various factors influencing disinfection. (RGPV Dec 2023)
Q.3 Define and classify disinfection. What are the various factors affecting disinfection? (RGPV June 2020)
Q.4 Write a detailed note on Viruses. (RGPV Nov 2019)
Q.5 Classify Disinfectants. Explain their mode of action with suitable examples. (RGPV Nov 2019)
UNIT 4 – Designing of Aseptic Area
Q.1 Describe various mode of action of disinfectants. (RGPV Dec 2023)
Q.2 Describe Laminar air flow with suitable diagram. (RGPV Dec 2023)
Q.3 Explain the principle and methods for standardization of vitamins. (RGPV Dec 2023)
Q.4 Give classification of clean area in detail. (RGPV Dec 2023)
Q.5 What are Microbiological assay? Discuss the principle and methods of microbiological assay of antibiotics. (RGPV June 2020)
Q.6 Discuss microbiological assay of antibiotics with suitable example. (RGPV Nov 2019)
UNIT 5 – Types of Spoilage
Q.1 Discuss factors affecting the microbial spoilage of pharmaceutical products. (RGPV Dec 2023)
Q.2 Describe the different methods of prevention of contamination in an aseptic area. (RGPV Dec 2023)
Q.3 Explain Food spoilage. Write a short note on food spoilage microbes. Discuss different technique of preservation of food. (RGPV June 2020)
Q.4 Define the general procedure for Cell culture. Briefly discuss about evaluation of microbial stability of various formulations. (RGPV June 2020)
Q.5 Explain Animal cell culture with their pharmaceutical applications. (RGPV Nov 2019)
Extra Questions
Q.1 Write short notes on any two (RGPV Dec 2023)
a) Ultra-structure of bacteria
b) Sterility indicators
c) Evaluation of bactericidal and bacteriostatic agents
d) Growth of animal cells in culture
Q.2 Write short notes on any three of the following. (RGPV June 2020)
a) History of microbiology
b) Staining techniques
c) Prokaryotes and Eukaryotes
d) Reproduction and nutritional requirement of viruses
Q.3 Write short notes on Any three (RGPV Nov 2019)
a) Factors affecting disinfection
b) Sterility testing
c) History and scope of microbiology
d) Microbial contaminants
— Best of Luck for Exam —
