B.Pharma Important Question
BP-701T,
Instrumental Methods of Analysis,
VII Sem, RGPV, B.Pharmacy
Unit-I UV Visible spectroscopy
Q.1 What is Quenching? Enumerate the various factors which influence quenching effect. (RGPV Dec 2024)
Q.2 Draw and explain with a neat label diagram of a double-beam LUV spectrophotometer. Explain various spectra photometric titrations with suitable graphs. (RGPV Dec 2024)
Q.3 Explain singlet, doublet, and triplet electronic states in fluorimetry. Mention the factors affecting fluorescence. (RGPV Dec 2024)
Q.4 Explain the Derivation of Beer Lambert’s Law. (RGPV Nov 2023)
Q.5 Explain Quenching and factors affecting Fluorescence intensity. (RGPV Nov 2023)
Q.6 Describe the principle and instrumentation of Infrared Spectroscopy. (RGPV Nov 2023)
Q.7 Discuss electronic transitions, Beer and Lambert’s law and applications of UV Visible spectroscopy. (RGPV Nov 2022)
Q.8 What are the concepts of singlet, doublet and triplet electronic states and quenching in Fluerimetry? (RGPV Nov 2022)
UNIT–II IR spectroscopy
Q.1 Explain how vibration frequency occurs in IR. Write factors affecting vibrations. (RGPV Dec 2024)
Q.2 Explain the types of molecular vibrations in Infrared spectroscopy. (RGPV Nov 2023)
Q.3 What are the advantages of Flame photometry? (RGPV Nov 2023)
Q.4 Write down the Principle and Instrumentation of Atomic absorption spectroscopy. (RGPV Nov 2023)
Q.5 Discuss modes of vibrations in poly atomic molecules, sample handling, factors affecting vibrations for IR spectroscopy. (RGPV Nov 2022)
UNIT–III Introduction to chromatography
Q.1 Write about the Guard column and its significance. Explore elution techniques in column chromatography. (RGPV Dec 2024)
Q.2 Define Rf, Rm, value. Add a note on detection methods in TLC. (RGPV Dec 2024)
Q.3 Explain the basic principle, Instrumentation and application of thin layer chromatography. (RGPV Nov 2023)
Q.4 Explain gel electrophoresis in detail. (RGPV Nov 2023)
Q.5 Enlist the advantages and disadvantages of Gel chromatography. (RGPV Nov 2023)
Q.6 Explain the different development techniques used in Paper chromatography. (RGPV Nov 2023)
Q.7 Explain the basic operations involved in TLC. (RGPV Nov 2023)
Q.8 Define chromatography and classify them. (RGPV Nov 2023)
Q.9 Describe principle, methodology, Rf values and applications of Thin layer chromatography. (RGPV Nov 2022)
Q.10 Discuss gel electrophoresis and factors affecting Electrophoresis mobility. (RGPV Nov 2022)
Q.11 What are the advantages, disadvantages of paper – chromatography? (RGPV Nov 2022)
UNIT–IV Gas chromatography
Q.1 What is derivatization? Explain various methods of derivatization in gas chromatography. (RGPV Dec 2024)
Q.2 What is two-dimensional paper chromatography? Discuss the methodology. (RGPV Dec 2024)
Q.3 Detailed the principle, instrumentation, and pharmaceutical applications of HPLC. (RGPV Dec 2024)
Q.4 Described the HPLC pump in detail. (RGPV Dec 2024)
Q.5 Explain the concept of plate theory and rate theory for increasing the efficiency of column in chromatography. (RGPV Dec 2024)
Q.6 Described the Instrumentation and working of Gas Chromatography. (RGPV Nov 2023)
Q.7 Described the HPLC pump in detail. (RGPV Nov 2023)
Q.8 Described the application of HPLC. (RGPV Nov 2023)
Q.9 Elaborate instrumentation of High Performance Liquid Chromatography (HPLC). (RGPV Nov 2022)
UNIT–V Ion exchange chromatography
Q.1 Mention the various factors affecting ion exchange chromatography. Classify and explain the ideal properties of ion exchange resins. (RGPV Dec 2024)
Q.2 Discuss the steps involved in size exclusion chromatography. (RGPV Dec 2024)
Q.3 What is the principle involved in affinity chromatography? Discuss applications of affinity chromatography. (RGPV Dec 2024)
Q.4 Explain the methodology of Affinity chromatography. (RGPV Nov 2023)
Q.5 What are the properties and mechanism of ion exchange process of Ion exchange chromatography? (RGPV Nov 2022)
Extra Questions
Q.1 Write notes on following: (RGPV Nov 2022)
a) Hospital formulary
b) Responsibilities and functions of hospital pharmacists.
c) Patient counseling
— Best of Luck for Exam —
