B.Pharma Important Question

BP-803T(E)/BP-804T(K)

Advanced Instrumentation Techniques,  

VIII Sem, RGPV, B.Pharmacy

Unit I Nuclear Magnetic Resonance Spectroscopy

Q.1 Write down the factors affecting chemical shift with a suitable example. (RGPV May 2024)

Q.2 Explain the instrumentation and working of the Mass Spectrometer.  (RGPV May 2024)

Q.3 What is Chemical shift? Elaborate factors affecting spin-spin coupling.  (RGPV May 2024)

Q.4 Describe the Principle, instrumentation and application of NMR. (RGPV May 2024)

Q.5 Explain the following. (RGPV May 2024)

a) Validation of Gas Chromatography

b) Fragmentation in Mass spectroscopy

Q.6 What is Chemical shift? Elaborate factors affecting spin spin coupling. (RGPV June 2020)

Q.7 Compare the principle and applications of H-NMR and C-NMR. (RGPV June 2020)

Q.8 What is the role of ionization techniques in Mass spectroscopy? Discuss the various detectors used along with its application in pharmaceutical industry. (RGPV June 2020)

Q.9 Explain the following.-Fragmentation in Mass spectroscopy. (RGPV June 2020)

Unit II Thermal Methods of Analysis

Q.1 Describe the principle of TGA and DTA methods.  (RGPV May 2024)

Q.2 What is the instrumentation and application of DSC? (RGPV May 2024)

Q.3 Describe the rotating crystal technique. (RGPV May 2024)

Q.4 Describe the principle of TGA and DTA methods.  (RGPV June 2020)

Q.5 What is the concept of rotating crystal techniques? How powder diffraction XRD is useful in structure elucidation of pharmaceutical compounds?  (RGPV June 2020)

Unit III Calibration and Validation

Q.1Write short notes on any two of the following. (RGPV May 2024)

a) Liquid-liquid extraction

b) HPTLC-MS

c) Flame Photometer.

Q.2 Compare calibration and validation. Briefly explain the process of calibration of UV spectrophotometer.  (RGPV June 2020)

Q.3 Explain the following.- Validation of HPLC.  (RGPV June 2020)

Unit IV Radio Immune Assay

Q.1 Describe the Importance, Principles, Methods, Limitations and Applications of Radioimmunoassay. (RGPV May 2024)

Q.2 Write short notes on any two of the following. (RGPV June 2020)

a) Solid phase extraction.

b) Radio-immuno assay.

c) HPTLC-MS.

Unit V Hyphenated Techniques

Q.1 Discuss the importance of hyphenated techniques in analysis with special reference to GC-MS/MS.  (RGPV May 2024)

Q.2 Discuss the importance of hyphenated techniques in analysis with special reference to LC-MS/MS.  (RGPV June 2020)

— Best of Luck for Exam —